H3N2 homoeopathic influenza virus solution modifies cellular and biochemical aspects of MDCK and J774G8 cell lines
Influenza viruses cause highly contagious acute respiratory illnesses with significant mortality, especially among young children, elderly people, and individuals with serious medical conditions. This encourages the development of new treatments for human flu. Biotherapies are diluted solutions prepared from biological products compounded following homoeopathic procedures. This study was aimed to develop a biotherapy prepared from the infectious influenza A virus (A/Aichi/2/68 H3N2) and to verify its in vitro response.
The methodology included was preparation of the ultradiluted influenza virus solution in the homoeopathic dilution 30x, which was termed Influenzinum RC. The cellular alterations induced by this preparation were analyzed by optical and electron microscopy, MTT and neutral red assays. Glycolytic metabolism (PFK-1) was studied by spectrophotometric assay. Additionally, the production of tumor necrosis factor-α (TNF-α) by J774.G8 macrophage cells was quantified by ELISA before and after infection with H3N2 influenza virus and treatment.
The results showed that Influenzinum RC did not cause cytotoxic effects but induced morphological alterations in Madin–Darby canine kidney (MDCK) cells. After 30 days, a significant increase (p < 0.05) in mitosis rate was detected compared to control. MDCK mitochondrial activity was changed after treatment for 10 and 30 days. Treatment significantly diminished (p < 0.05) PFK-1 activity. TNF-α in biotherapy-stimulated J774.G8 macrophages indicated a significant (p < 0.05) increase in this cytokine when the cell supernatant was analyzed.
The authors have concluded that the Influenzinum RC altered cellular and biochemical features of MDCK and J774G8 cells.
Influenzinum is available from Schwabe India from 30CH to CM. Many physicians like to use Alpha-WD or Echinacea angustifolia 1x tablets simultaneously as immune stimulant.
Reference: Camila Monteiro Siqueira, Beatriz Costa, Ana Maria Amorim, Marta Gonçalves, Venício Féo da Veiga, Morgana Castelo-Branco, Christina Takyia, Patrícia Zancan, Fernando Portela Câmara, José Nelson Couceiro, Carla Holandino, H3N2 homeopathic influenza virus solution modifies cellular and biochemical aspects of MDCK and J774G8 cell lines, Homeopathy, Volume 102, Issue 1 , Pages 31-40, January 2013
Influenza viruses cause highly contagious acute respiratory illnesses with significant mortality, especially among young children, elderly people, and individuals with serious medical conditions. This encourages the development of new treatments for human flu. Biotherapies are diluted solutions prepared from biological products compounded following homoeopathic procedures. This study was aimed to develop a biotherapy prepared from the infectious influenza A virus (A/Aichi/2/68 H3N2) and to verify its in vitro response.
The methodology included was preparation of the ultradiluted influenza virus solution in the homoeopathic dilution 30x, which was termed Influenzinum RC. The cellular alterations induced by this preparation were analyzed by optical and electron microscopy, MTT and neutral red assays. Glycolytic metabolism (PFK-1) was studied by spectrophotometric assay. Additionally, the production of tumor necrosis factor-α (TNF-α) by J774.G8 macrophage cells was quantified by ELISA before and after infection with H3N2 influenza virus and treatment.
The results showed that Influenzinum RC did not cause cytotoxic effects but induced morphological alterations in Madin–Darby canine kidney (MDCK) cells. After 30 days, a significant increase (p < 0.05) in mitosis rate was detected compared to control. MDCK mitochondrial activity was changed after treatment for 10 and 30 days. Treatment significantly diminished (p < 0.05) PFK-1 activity. TNF-α in biotherapy-stimulated J774.G8 macrophages indicated a significant (p < 0.05) increase in this cytokine when the cell supernatant was analyzed.
The authors have concluded that the Influenzinum RC altered cellular and biochemical features of MDCK and J774G8 cells.
Influenzinum is available from Schwabe India from 30CH to CM. Many physicians like to use Alpha-WD or Echinacea angustifolia 1x tablets simultaneously as immune stimulant.
Reference: Camila Monteiro Siqueira, Beatriz Costa, Ana Maria Amorim, Marta Gonçalves, Venício Féo da Veiga, Morgana Castelo-Branco, Christina Takyia, Patrícia Zancan, Fernando Portela Câmara, José Nelson Couceiro, Carla Holandino, H3N2 homeopathic influenza virus solution modifies cellular and biochemical aspects of MDCK and J774G8 cell lines, Homeopathy, Volume 102, Issue 1 , Pages 31-40, January 2013